By combining lineage information with single-cell RNA sequencing profiles, we recapitulate canonical developmental relationships between different tissue types and reveal the nearly complete transcriptional convergence of endodermal cells of extra-embryonic and embryonic origins. Farrell, J.

This is an important step that has broad implications for the scientific community.” Cells receive and process information much like computers. Understanding the emergence of complex multicellular organisms from single totipotent cells, or ontogenesis, represents a foundational question in biology.

Haapaniemi, E., Botla, S., Persson, J., Schmierer, B. Zachary … Shah, S., Lubeck, E., Zhou, W. & Cai, L. In situ transcription profiling of single cells reveals spatial organization of cells in the mouse hippocampus. & Taipale, J. CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response. 1019703 Contact details. San Francisco, then a postdoc in David Altshuler’s lab (human genetics and genomics) at MGH and the Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain. & van Oudenaarden, A. Whole-organism clone tracing using single-cell sequencing. You can also search for this author in This protein, called IFNB1 (for Interferon beta 1) requires precise control: if a virus is present, it must be highly active, yet if bacteria are the offending agents, its activity should be minimized. CRISPR/Cas9 cleavages in budding yeast reveal templated insertions and strand-specific insertion/deletion profiles. Fincher, C. T., Wurtzel, O., de Hoog, T., Kravarik, K. M. & Reddien, P. W. Cell type transcriptome atlas for the planarian Plass, M. et al. Overview Financials Documents People Operations Income. Dendritic cells are among the first to detect pathogens and can differentiate one type of pathogen from another, allowing them to orchestrate a pathogen-specific immune response.

is a Gordon and Betty Moore fellow of the Life Sciences Research Foundation. Schimmel, J., Kool, H., van Schendel, R. & Tijsterman, M. Mutational signatures of non-homologous and polymerase theta-mediated end-joining in embryonic stem cells. Briggs, J. Moreover, 12 of the regulators identified in the Founded by MIT, Harvard and its affiliated hospitals, and the visionary Los Angeles philanthropists Eli and Edythe L. Broad, the Broad Institute includes faculty, professional staff and students from throughout the MIT and Harvard biomedical research communities and beyond, with collaborations spanning over a hundred private and public institutions in more than 40 countries worldwide. “We now have a detailed view of the circuitry that controls this critical process, providing a deeper understanding of immune biology that could inspire novel ways to treat disease and design better vaccines." Ibarra-Soria, X. et al. You can also search for this author in

and D.Y. Kalhor, R. et al. T.M.N. To figure out how these potential regulators work and how they are wired together, the researchers systematically eliminated each of them, and recorded the changes in the circuit’s output.

Broad Fellows. Another remarkable finding is the way these regulators operate. prepared several sequencing and transposon libraries and were engaged in discussion. and JavaScript.Ontogeny describes the emergence of complex multicellular organisms from single totipotent cells. & Liu, D. R. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. You can also search for this author in Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo. conducted experiments, and M.M.C.