For this purpose, protein transfer onto the surface of polyacrylamide hydrogels must result in geometrically well-resolv...Mutation of highly conserved residues in transcription factors may affect protein-protein or protein-DNA interactions, leading to gene network dysregulation and human disease. The water contact angle of substrates used in μCP differs substantially from average of 111° for PDMS (n = 12 measurements) to approximately 0° for Hellmanex-cleaned glass (the substrate used for μCP). Scale bar is 45 μm wide. In typical micropost assays, simple 2-point axial measurements are impossible since cells are suspended across 20-50 posts at a time. Three channels are shown (gelatin for protein patterning, phase for cell outline, and LifeAct-GFP for actin structures). Search for more papers by this author . (MP4)Single MDCK on μCP gel. We review the deve...During metastasis, melanoma cells must be sufficiently deformable to squeeze through extracellular barriers with small pore sizes. By setting a threshold of a 0.84 correlation coefficient, the LOP protocol resulted in more acceptable features than μCP (highlighted in green). There is no approved therapy. (MP4)Lamellipodia beyond pattern edge for a cell on LOP gel.

Here, iPS-derived cardiomyocytes from subjects with a heterozyg...Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are a powerful platform for uncovering disease mechanisms and assessing drugs for efficacy/toxicity. Scale bar is 45 μm wide. Here, to determine the direct role of the nucleus itself in converting forces to changes in gene expression, also...Mechanotransduction of extracellular stimuli into the chromatin located in the cell nucleus and overall cell mechanics may require the physical support of structures of A-type lamins. Microphysiological systems (MPS), also known as organs‐on‐a‐chip, are a class of microfluidic platforms that recreate properties of tissue microenvironments. Detection of such effects earlier in drug research using simpler, in vitro tes...Background: We measured the contact angle of PLL-g-PEG coated glass before and after polymerizing a polyacrylamide gel. Numerous genetic diseases and cancers have alterations in nuclear morphology, and methods for characterization of morphology could aid in both diagnoses and fundamental understanding of these disorders. WHAT IS THE ADVANTAGE OF JOINING THE OoC COMMUNITY ?ACCESS YOUR PERSONAL SPACE, FORUM AND MEMBER FEATURES The image is a ‘straightened’ path along the cell edge (on bottom) extending 10 μm past the protein pattern (away from the cell). Here is presented a study of ch...Injection of stem cells for regenerative therapies requires optimal pressure to efficiently deliver cells, but inadequate designs of devices for injection can generate elevated stresses on cells resulting in altered cell response and cell death. Studies of allele-specific silencing in hypertrophic cardiomyopathy (HCM) to date have focused on mouse models of disease. Xinning Yang. However, while their myogenic maturity increases with time, they do not resemble adult cardiomyocytes in their morphology, structural organization, mechanical output...Microscale and nanoscale structures can spatially pattern endothelial cells (ECs) into parallel-aligned organization, mimicking their cellular alignment in blood vessels exposed to laminar shear stress. Alexandre Ribeiro (born January 20, 1981) is a Brazilian Jiu-Jitsu practitioner, mixed martial artist and submission wrestler. We visualize and quantify deformability of single cells using micropipette aspiration and examine the migration potential of a population of melanoma cells using a flow migration apparatus. 10:50 – 11:10 Micropatterned Human iPSC-derived Cardiomyocytes Alexandre Ribeiro (FDA) 11:10 – 11:30 Using Adult Human Primary Cardiomyocyte Models Three separate time-lapse acquisitions (5 minute increments, time shown at upper left) of single MDCK cells on LOP-functionalized 25 kPa PAAm gels. Cellular structure can be analyzed from microscopy imaging data. Tissues also remodel in both development and disease, changing their mechanical properties. The structure, organization, and mechanical properties of the different regions of the nucleus – the nuclear envelope, the nucleoskeleton, and the nucleoplasm and genome – are presented individually and in context with one another. Recently, we developed a functional human and pig heart slice biomimetic culture system that fully preserves the viability and functionality of 300 micrometer hea...Contractility of the myocardium engines the pumping function of the heart and is enabled by the collective contractile activity of its muscle cells: cardiomyocytes. The average water contact angle is similar with 27° for PLL-g-PEG glas...Single MDCK on LOP gel. Email : Alexandre.Ribeiro@fda.hhs.gov. We used β-adrenergic receptor (β-AR)...Single cardiomyocytes contain myofibrils that harbor the sarcomere-based contractile machinery of the myocardium. Culturing cardiomyocytes on arrays of elastomeric microposts can enable inexpensive and high-throughput studies of heart disease at the single-cell level. (MP4)Lamellipodia beyond pattern edge for a cell on μCP gel. Three channels are shown (gelatin for protein patterning, phase for cell outline, and LifeAct-GFP for actin structures). A time-lapse acquisition (5 minute increments, time shown at upper left) of an MDCK cell lamellipodia extending past the protein pattern region created by LOP. The image is a ‘straightened’ path along the cell edge (on bottom) extending 10 μm past the protein pattern (away from the cell). Phone : Adress : - Namely, short working distances between the objective and focal plane, a moving focal plane due to Poisson's contraction with stretching, and issues of optical transparency or non-matching refractive indices. We selected 389–416 features for each gel sample and then performed cross correlation analysis on the collected feature montage. Human-induced pluripotent stem cells (hiPSCs) can be differentiated into CMs (hiPSC-CMs) that model cardiac contractile mechanical output more robustl...Polyacrylamide gels functionalized with extracellular matrix (ECM) proteins are commonly used as cell culture platforms to evaluate the combined effects of ECM composition, cell geometry and substrate rigidity on cell physiology. Research Project Title.